Immunoregulation of Bone Marrow-Derived Mesenchymal Stem Cells on the Chronic Cigarette Smoking-Induced Lung Inflammation in Rats

Figure 3

Flow cytometry analysis of the frequency of different subsets of CD4+ T cells in the lungs of rats. The lung-infiltrated lymphocytes were isolated from individual rats and stimulated with PMA/ionomycin for 5 hours. After being stained, the cells were first gated on lymphocytes and then gated on CD4+ T subsets. Subsequently, the percentages of CD4+IL-17+ Th17, CD4+CD25+Foxp3+ Tregs, CD4+IFN-γ+ Th1, and CD4+IL-4+ Th2 cells in total CD4+ T cells were analyzed. Data are representative FACS charts and expressed as the means ± SD of individual groups of rats ( per group) from four independent experiments. (a) Flow cytometry analysis. (b) Quantitative analysis. , versus the controls; , versus the BMSCs.

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