Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent Signaling Pathways

Figure 7

IP₃ pathway inhibition impairs Ang-1-induced capillary-like formation in vitro. Cells were plated in Matrigel-coated dishes and incubated in EBM-2 + 2% FBS for 5 h in the presence or absence of Angs or/and inhibitors as indicated. Each condition was tested in triplicate. Representative images of one of three independent experiments (Ang-1: a, b; Ang-2: d, e). Quantitative evaluation of tube formation as the number of closed polygons formed in seven fields for each experimental condition (c, f). Data in bar charts represent mean ± s.e.m. of three independent experiments. by Student’s -test.