Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells

Figure 5

Glutamate uptake in starved Müller cells. Müller cells were cultured 24 hours in energy sufficient conditions or starved within the same time frame. Kinetic L-glutamate uptake at extracellular glutamate concentrations from 1 to 500 μM was measured during the described conditions. Starvation significantly increased glutamate uptake ((a); and . at 500 μM glutamate). The relative maximal uptake rate, , was also significantly increased ((b); ). Values are represented as mean values ± SEM of 5 experiments. Differences were tested using unpaired two-tailed -test at the different glutamate concentrations.

(a)

(b)