Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana

Figure 3

LRB7 expression and TIP2 abundance in leaves (L), stems (S), and roots (R) of C. bungeana. (a) Semiquantitative RT-PCR showed LRB7 expressed exclusively in roots, M: DL2000 marker. (b) Quantitative experiment for total proteins from the cell lysates of E. coli BL21 (DE3) pLysS transformed with PGEX-4T-3-LRB7. M: unstained protein molecular weight marker; CK1: cell lysates without induction with 20% IPTG; CK2: cell lysates inducted with 20% IPTG; 1–5: total proteins of the cell lysates of E. coli BL21 (DE3, induced by 20% IPTG) cultured at 2, 3, 4, 5, and 6 hr, respectively. (c) Western blot analysis of TIP2. The anti-LRB7 specifically recognized 25 kD band in C. bungeana roots (R), but not in stems (S) or leaves (L).

(a)

(b)

(c)