Percutaneous Transluminal Angioplasty in Patients with Peripheral Arterial Disease Does Not Affect Circulating Monocyte Subpopulations

Figure 1

Flow cytometric analysis of monocytes and their subsets from PBMC. Monocytes with a part of lymphocytes (b) were gated according to forward scatter (FSC) and side scatter (SSC) from peripheral blood mononuclear cells (PBMCs (a)). Subsequently, cells were gated in an HLA-DR/CD14 plot to exclude HLA-DR-negative Natural Killer cells (c). Finally, monocyte subpopulations were identified according to CD14 and CD16 expressions (CD14++CD16−, CD14++CD16+, and CD14+CD16++ (d)). Percentages of major monocyte subpopulations are indicated (d) in Sample 0 (before PTA) and Sample 1 (after PTA).

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