Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment

Table 2

Carbon source metabolic characteristics of SP1 in GN III microplate.

Carbon source reactions	SP1
Polymers
Dextrin	/
Glycogen	−
Tween 40	///
Sugars and sugar derivatives
N-Acetyl-D-galactosamine	−
N-Acetyl-D-glucosamine	/
N-Acetyl-β-D-mannosamine	−
D-Arabitol	−
D-Cellobiose	−
D-Fructose	/
D-Fucose	/
D-Galactose	/
Gentiobiose	−
α-D-Glucose	/
3-Methyl-glucose	−
Myoinositol	−
α-D-Lactose	−
D-Salicin	−
D-Maltose	/
D-Mannitol	/
D-Mannose	−
D-Melibiose	−
β-Methyl-glucoside	/
Stachyose	−
D-Raffinose	/
L-Rhamnose	−
D-Sorbitol	−
Sucrose	+
D-Trehalose	/
D-Turanose	/
Methyl esters
Methyl pyruvate	///
D-Lactic acid methyl ester	−
Carboxylic acids
Acetic acid	−
Acetoacetic acid	/
Citric acid	−
Formic acid	/
L-Galactonic acid lactone	/
D-Galacturonic acid	/
D-Gluconic acid	/
D-Malic acid	−
L-Malic acid	+
D-Glucuronic acid	/
α-Hydroxy-butyric acid	−
β-Hydroxy-D,L butyric acid	/
ρ-Hydroxy-phenylacetic acid	−
D-Saccharic acid	−
Mucic acid	/
Carboxylic acids
α-Keto butyric acid	−
α-Keto glutaric acid	−
L-Lactic acid	/
Propionic acid	−
Quinic acid	+
Bromosuccinic acid	/
Amides
Glucuronamide	//
Amino acids, peptides, related chemicals
L-Alanine	+
L-Aspartic acid	+
L-Glutamic acid	/
L-Histidine	/
Glycyl-L-proline	−
L-Pyroglutamic acid	/
D-Serine	−
L-Serine	−
D-Aspartic acid	−
L-Arginine	/
γ-Amino butyric acid	/
Nucleosides
Inosine	−
Alcohols
Glycerol	//
D-Glucose-6-phosphate	−
D-Fructose-6-phosphate	//
Else
pH 5	+
pH 6	+
1% NaCl	+
4% NaCl	/
8% NaCl	/
1% sodium lactate	+
Fusidic acid	−
Troleandomycin	−
Rifamycin SV	−
Minocycline	−
Lincomycin	−
Guanidine HCl	−
Niaproof 4	−
Vancomycin	−
Tetrazolium violet	/
Tetrazolium blue	−
Nalidixic acid	−
Lithium chloride	/
Potassium tellurite	+
Aztreonam	/
Sodium butyrate	/
Sodium bromate	−

Note. +: positive response; −: negative response; /: borderline; //: mismatched positive; and ///: mismatched negative.