Effect of Phosphatase and Tensin Homologue on Chromosome 10 on Angiotensin II-Mediated Proliferation, Collagen Synthesis, and Akt/P27 Signaling in Neonatal Rat Cardiac Fibroblasts

Figure 1

PTEN overexpression in cardiac fibroblasts (CFs) by adenoviral gene transfer. Infection with Ad-GFP or Ad-PTEN for 48 h. (a, b) PTEN protein expression was assessed by western blot analysis. (c) The PTEN mRNA level was assessed by semiquantitative RT-PCR. Each experiment was repeated three times with 3-4 replicates each. , compared with the control Ad-GFP and uninfected groups.

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