Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease

Figure 2

Expressions of miR-425a and miR-23a represent the hemolysis status of clinical samples. The qPCR analysis revealed that expressions of miR-451a and miR-23a stored at (a) RT or (b) at 4°C were stable from 0 h to 8 h. The Ct values shown in the tables indicate the qPCR results for five individuals at different time points. The value indicates the significance of each time point compared with time 0 h. (c) Manual hemolysis test was performed in 32 differential hemolytic plasma samples (see Materials and Methods). Hemolytic grade was defined by hemolysis card (Supplementary Fig.  1A). The miR-425a/miR-23a ratio increased as hemolysis grade increased.

(a)

(b)

(c)