Cloning and Expression of the γ-Polyglutamic Acid Synthetase Gene pgsBCA in Bacillus subtilis WB600

Figure 3

Map of electrophoresis of recombinant plasmid pWB980-pgsBCA after digestion. Note: Lane 1, pgsBCA PCR product; Lane 2, after double digestion of pWB980-pgsBCA with BamHI and HindIII; Lane M, DNA markerIII (Tiangen).