Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples

Table 1

Sequences of RPA and PCR primers and probes used in the study.

Assay	Description	Sequence
RPA	RR2Y probe	cccgccttcagtttaaactatcagtgtttggagc-T(BHQ-2)-t-dSpacer-a-T(TAMRA)-aaccacgattgaag
	RR2Y forward primer	ccctcttggcttttctaagtttgagctcgttactg
	RR2Y reverse primer	cccgccttcagtttaaactatcagtgtttgg
	lec probe	ggaaactgtttctttcagctggaacaag-T(FAM)-t-dSpacer-g-T(BHQ-1)-gccgaagcaacc
	lec forward primer	ccagaatgtggttgtatctctctccctaacctt
	lec reverse primer	cccgaggaggtcacaatagcgtctccttggag
PCR	RR1 forward primer	tttgggaccactgtcggcagaggcatctt
	RR1 reverse primer	gatttgaattcagaaccttgtgca
	RR2Y forward primer	tcccgctctagcgcttcaat
	RR2Y reverse primer	tcgagcaggacctgcagaa
	lec forward primer	gtttgacactttccggaactcttg
	lec reverse primer	ctgtcacatttagatggcctcatg

BHQ = dT Black Hole Quencher; TAMRA = dT TAMRA; FAM = dT FAM.