Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration

Figure 1

LAB setup and cell patterns. (a) The LAB setup: (1) laser beam, (2) donor gold-coated slide to generate the jet (the cell-containing solution is facing (3)), and (3) receptor collagen-coated slide. The red dots in (3) represent the cell islets on the collagen hydrogel postprinting. (b) Cell spots were printed at each 250 μm in a given line segment and consecutive line segments were separated by either 500 μm or 1000 μm. (c) Examples of HBMSCs postprinting visualized by bright field view (laser energy 25 μJ) and tdTomato-expressing HUVECs in epifluorescence (laser energy 20 μJ). (d) Mean distances between spot centers () and between segments ( for each distance), measured on the collector slide with ImageJ®.

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