The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis

Figure 6

Bone marrow-derived α-SMA-positive cells in the pancreas from cerulein treated female mice that received either a wild-type (WT) or an IL-10 knock out (KO) male bone marrow (BM) transplant. (a) ISH for the Y chromosome in the spleen of female mice that received a WT male BM transplant. (b) IHC for α-SMA (red) and ISH for the Y chromosome shows BM-derived myofibroblasts in the pancreas from a mouse that received a WT male BM transplant (arrow, 400x) ((c) and (d)). Active myofibroblasts illustrated by α-SMA expression (red), while a small brown dot in the nucleus indicates this is a BM-derived myofibroblast in the pancreas from a mouse that received an IL-10 KO BM transplant (arrow). (e) The number of myofibroblasts per high power field (HPF) in mice receiving either a WT BM (blue bars) or IL-10 KO BM (red bars) in both the chronic and recovery groups. (f) The percentage of BM-derived myofibroblasts in the same groups.

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