The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis

Figure 7

Immunohistochemical staining with F4/80 for macrophages in the pancreas from cerulein treated female mice that received either a wild-type (WT) male bone marrow (BM) or an IL-10 knock out (KO) male BM transplant. (a) and (b) show that in saline-treated mice (control groups), the F4/80 positive cells are rare in the pancreas of WT male BM transplanted mice (a), while the infiltration of F4/80-positive cells is increased in the pancreas of IL-10 KO male BM transplanted mice (b). (c) and (d) show that macrophage infiltration is significantly increased in the pancreas of both WT BM transplanted (c) and IL-10 KO BM (d) transplanted mice after receiving 6 weeks of cerulein injections (chronic groups). (e) and (f) show that macrophage infiltration was less in the WT BM transplanted mice after a 3-week recovery (e) but was unaltered in the IL-10 KO BM transplanted mice (f). (g) shows F4/80 staining in the spleen from a mouse that received a WT BM transplant but no cerulein. (h) shows the number of macrophages per HPF in mice receiving either a WT BM (blue bars) or an IL-10 KO BM (red bars).

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