Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

Figure 3

The biological characteristics of immortalized STCs were found to be similar to that of primary STCs. (a) Immunofluorescence staining of primary STCs for cell keratin 7 expression (1) and hTERT-STCs at passage 50 (2) was positive. Immunofluorescence staining of hTERT-STCs for PBS was (3) (scale bars, 50 μm). (b) The secretion level of primary STCs and hTERT-STCs at passage 50 CG-β, HEK293T cells (negative control) (). (c) The secretion level of primary STCs and hTERT-STCs at passage 50 PL, HEK293T cells (negative control) (). (d) The expression of enJSRV-env and syncytin-Rum1 was detected by RT-PCR. Lane 1 was Mongolian sheep genomic DNA; lane 2 was primary STCs; lane 3 was hTERT-STCs at passage 50; and lane 4 was negative control. Lanes 5–8 are the same as in lanes 1–4. hTERT-STCs: human telomerase reverse transcriptase-sheep trophoblast cell line. RT-PCR: reverse transcriptase polymerase chain reaction.

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