Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response

Figure 3

PTEN decreased LPS-induced Akt activity. (a) RAW264.7 cells were transfected with 8 μg of CMV or wild-PTEN with Lipofectamine 2000. About twenty hours after transfection, culture medium was changed to the serum-free RPMI 1640 for another 8–12 hours, cells were treated with LPS (1 μg/mL) for 0, 15, and 30 min, and then cell lysate was harvested. p-Akt and the total protein levels were detected accordingly by western blot. (b) RAW264.7 cells were left untreated or treated with the bpV(Pic) 100 nM for 1 hour, followed by stimulation of LPS (1 μg/mL) for 0, 10, 20, 30, 45, and 60 min; then, cell lysate was harvested and p-Akt and the total protein levels were detected accordingly by western blot. Compared with the cells transfected with CMV, LPS-induced phosphorylation of Akt was significantly inhibited in the cells transfected with wild-type PTEN () (a). On the contrary, LPS-induced phosphorylation of Akt was significantly increased in the presence of bpV(Pic) (, ) (b). ; .

(a)

(b)