MP Resulting in Autophagic Cell Death of Microglia through Zinc Changes against Spinal Cord Injury

Figure 2

MP reduced the activation of microglia in SCI rats. After 12 hours and 18 hours treatment, spinal cord was collected for histological analysis, ELISA and RT-PCR. (a) Immonoexpression of Iba1 (brown) in spinal cord at 12 h and 18 h. Scale bar = 200 μm, 50 μm; (b), (c) Spinal cord around lesion was subjected to examine the expression of iNOS, IL-6, IL-10, LC-3B and Beclin-1 by RT-PCR. Data were expressed as mean ± SD ( for each group). , versus normal group; , versus SCI group.

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