Cornel Iridoid Glycoside Improves Locomotor Impairment and Decreases Spinal Cord Damage in Rats

Figure 6

Effects of CIG on expression of Nogo-A, p75NTR, and ROCKII in injured spinal cord. The immunohistochemical and stereological methods were used to measure the expression of Nogo-A, the p75 neurotrophin receptor (p75NTR), and Rho-associated coiled-coil forming protein kinase II (ROCKII) on the serial and equal interval slices. (a) The lesion epicenter (LEp) and adjacent area stained by immunohistochemical method. Scale bar = 500 m. (b) The LEp and adjacent area analyzed by stereological method. The representative images of Nogo-A (c), p75NTR (f), and ROCKII (i) positive cells were shown, respectively. Scale bar = 50 m. (d, g, j) The ratio of the number of Nogo-A, p75NTR, and ROCKII positive cells in the LEp to that in the total volume, respectively. (e, h, k) The number of Nogo-A, p75NTR, and ROCKII positive cells around the LEp (within 3 mm from the outmost edge of LEp), respectively. Data are expressed as mean ± SEM from 3 rats in each group. , , CIG-treated groups versus the SCI model group. Because there was no LEp in the sham control rats, the boundary of LEp could not be distinguished; thus the sham control group was absent in this figure.

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