Partial Portal Vein Arterialization Attenuates Acute Bile Duct Injury Induced by Hepatic Dearterialization in a Rat Model

Figure 1

Surgical procedures. (a) Bile duct ischemia (BDI) was induced as follows: The hepatic artery (HA) was ligated and divided. The common bile duct (CBD) was cannulated with a polyethylene tube and doubly ligated to interrupt the peribiliary plexus. (b) BDI was followed by partial portal vein arterialization (PPVA), which was performed as follows: The gastroduodenal artery (GDA) was divided. A piece of silk suture with a small needle was reserved at the proximal end of the GDA. The dorsal common HA and the left edge of the portal vein (PV) wall were sutured together using two pieces of 8-0 prolene sutures (white arrow). The reserved needle along with the GDA was guided between the two sutures into the PV lumen and out from the opposite side. The GDA retracted into the PV after the surplus end of the GDA was excised. Hemostasis was achieved using a suture (black arrow). The PV was clamped for <5 min during the whole procedure.

(a)

(b)