Dhcr7 Regulates Palatal Shelf Fusion through Regulation of Shh and Bmp2 Expression

Figure 4

Shh gene mRNA (left image and graph) and protein (right image and graph) expression analysis. A: control group; B: Dhcr7-siRNA inhibited group; C: supplemental cholesterol group. RT-PCR detection indicates that the difference was statistically significant when group B was compared with group A (); the difference was also statistically significant when group C was compared with group B (). WB analysis showed that the ratio of Shh/GAPDH difference was statistically significant when group B was compared with group A (); the difference was statistically significant when group C was compared with group B ().