Dhcr7 Regulates Palatal Shelf Fusion through Regulation of Shh and Bmp2 Expression

Figure 5

Bmp2 gene mRNA (left) and protein (right) expression analysis. A: control group; B: Dhcr7-siRNA inhibited group; C: supplemental cholesterol group. RT-PCR detection indicated that the difference was statistically significant when group B was compared with group A (); The difference was also statistically significant when group C was compared with group B (). WB analysis showed that the ratio of Bmp2/GAPDH difference is statistically significant when group B is compared with group A (); the difference is statistically significant when group C compared with group B ().