Dietary Fructose Activates Insulin Signaling and Inflammation in Adipose Tissue: Modulatory Role of Resveratrol

Table 1

Primer sequences of IR, IRS-1, IRS-2, Akt, PI3K, eNOS, SIRT1, mTOR, PPAR, Nrf2, NFB, iNOS, and internal standard GAPDH used for the mRNA expression determination of qRT-PCR.

Gene	Forward primer sequence (	Reverse primer sequence ()	Product; length (bp)	Gene bank; accession number
IR	GTGCTGCTCATGTCCTTAGA	AATGGTCTGTGCTCTTCGTG	234	XM_006248753.2
IRS-1	GCCAATCTTCATCCAGTTGC	CATCGTGAAGAAGGCATAGG	337	NM_012969.1
IRS-2	CTACCCACTGAGCCCAAGAG	CCAGGGATGAAGCAGGACTA	151	NM_001168633.1
Akt	GAAGAAGAGCTCGCCTCCAT	GAAGGAGAAGGCCACAGGTC	211	NM_033230.2
PI3K	ATGCAACTGCCTTGCACATT	CGCCTGAAGCTGAGCAACAT	320	NM_053481.2
eNOS	TGCACCCTTCCGGGGATTCT	GGATCCCTGGAAAAGGCGGT	189	XM_006235872.1
SIRT1	CGGTCTGTCAGCATCATCTTCC	CGCCTTATCCTCTAGTTCCTGTG	136	XM_008772947.1
mTOR	GCAATGGGCACGAGTTTGTT	AGTGTGTTCACCAGGCCAAA	94	NM_019906.1
PPARγ	CTCAGGTCAGAGTCGCCCC	GAGAGAGACCTCGTCAGGCT	205	NM_001145367.1
Nrf2	GATTCGTGCACAGCAGCA	GCCAGCTGAACTCCTTAGAC	466	XM_006234397.2
NFB	GGGTCAGAGGCCAATAGAGA	CCTAGCTTTCTCTGAACTGCAAA	71	AF_079314.1
iNOS	CTTCAGGTATGCGGTATTGG	CATGGTGAACACGTTCTTGG	352	XM_006246949.2
GAPDH	TGATGACATCAAGAAGGTGGTGAAG	TCCTTGGAGGCCATGTGGGCCAT	240	NM_017008.4