Silver-Containing Hydroxyapatite Coating Reduces Biofilm Formation by Methicillin-Resistant Staphylococcus aureus In Vitro and In Vivo

Figure 2

Box-and-whisker plots of biofilm coverage rates (BCRs) of implants after bacterial culture for 7 days and 14 days in vitro. In the 7-day experiment, 49 images of HA coating, including 14 images of 0.5% Ag-HA, 14 images of 1% Ag-HA, and 21 images of 3% Ag-HA, were used. In the 14-day experiment, 63 images of HA and 21 images of each group of Ag-HA were used. denotes significant differences between the BCRs of HA and each concentration of Ag-HA. The significance levels are as follows: 7 days: 0.5% Ag-HA, ; 1% Ag-HA, ; and 3% Ag-HA, ; 14 days: 0.5% Ag-HA, ; 1% Ag-HA, ; and 3% Ag-HA, . † denotes significant differences between the BCRs of 3% Ag-HA and other concentrations (0.5%, 1%) of Ag-HA. The significance levels are 0.5% Ag-HA, , and 1% Ag-HA, .