¹²⁵I Seeds Radiation Induces Paraptosis-Like Cell Death via PI3K/AKT Signaling Pathway in HCT116 Cells

Figure 5

After exposure to 2 Gy of ¹²⁵I seeds radiation the expression of AIP1 decreased. Cells lysates were harvested 48 h after exposure to the 0 Gy, 0.5 Gy, 1 Gy, and 2 Gy of ¹²⁵I seeds radiation. The protein expressions of AIP1 and caspase 3 were determined by western blotting. (a) Equal amounts of cell lysates from control and irradiated cells were analyzed. The expressions of AIP1 and caspase 3 were analyzed by western analysis with anti-AIP1 and anti-caspase 3 antibody. -Actin was evaluated as a loading control. (b)–(d) The expression of AIP1 (b), caspase 3 (c), and cleaved caspase 3 (d) was quantified using Photoshop software. The data are normalized to the β-actin and presented as means ± SD, .

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