Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin

Table 1

Primers used for detection of exotoxins and MSCRAMM using conventional PCR.

Proteins targeted	Primer forward (5′-3′)	Primer reverse (5′-3′)		Expected band size (bp)	Reference
Cna; Collagen binding protein	AAA GCG TTG CCT AGT GGA GA	AGT GCC TTC CCA AAC CTT TT	50°C	192	[1]
ClfA; Clumping factor A	CGC CGG TAA CTG GTG AAG CT	TGC TCT CAT TCT AGG CGC ACT T	55°C	314	[27]
ClfB; Clumping factor B	ATG ATC TTG CTT GCG TT	CCG ATT CAA GAG TTA CAC C	47°C	215	[27]
Spa; Protein A	TCA AGC ACC AAA AGA GGA AGA	GTT TAA CGA CAT GTA CTC CGT TG	51°C	Variable	[28]
FnBPA; Fibronectin binding protein A	GCG GAG ATC AAA GAC AA	CCA TCT ATA GCT GTG TGG	48°C	1279	[29]
FnBPB; Fibronectin binding protein B	GGA GAA GGA ATT AAG GCG	GCC GTC GCC TTG AGC GT	56°C	820	[29]
Bbp; Bone sialoprotein binding protein	AAC TAC ATC TAG TAC TCA ACA ACA G	ATG TGC TTG AAT AAC ACC ATC ATC T	53°C	575	[30]
IsdA; Iron regulated surface determinant A	CTG CGT CAG CTA ATG TAG GA	TGG CTC TTC AGA GAA GTC AC	52°C	332	[25]
IsdB; Iron regulated surface determinant B	ACG AGA GTT TGG TGC GCT AT	GTT GAG GCC CCT ACT TCT GA	55°C	192	This study
SdrD; Serine aspartate repeat gene D	CGG AGC TGG TCA AGA AGT AT	TGC CAT CTG CGT CTG TTG TA	52.3°C	500	[25]
SdrE; Serine aspartate repeat gene E	AGA AAG TAT ACT GTA GGA ACT G	GAT GGT TTT GTA GTT ACA TCG T	50°C	433	[31]
TSST-1; Toxic shock syndrome toxin	ACC CCT GTT CCC TTA TCA TC	TTT TCA GTA TTT GTA ACG CC	53°C	326	[32]
ETA; Exfoliative toxin A	GCA GGT GTT GAT TTA GCA TT	AGA TGT CCC TAT TTT TGC TG	58°C	93	[33]
ETB; Exfoliative toxin B	ACA AGC AAA AGA ATA CAG CG	GTT TTT GGC TGC TTC TCT TG	58°C	226	[33]
Hla; Alpha toxin	GTA CTA CAG ATA TTG GAA GC	GTA ATC AGA TAT TTG AGC TAC	47°C	274	[34]
Hlb; Beta toxin	GCC AAA GCC GAA TCT AAG	CGC ATA TAC ATC CCA TGG C	51°C	840	[29]
SEA; Staphylococcal enterotoxin A	TTG GAA ACG GTT AAA ACG AA	GAA CCT TCC CAT CAA AAA CA	50°C	120	[35]
SEB; Staphylococcal enterotoxin B	TCG CAT CAA ACT GAC AAA CG	GCA GGT ACT CTA TAA GTG CC	50°C	478	[35]
SEC; Staphylococcal enterotoxin C	GAC ATA AAA GCT AGG AAT TT	AAA TCG GAT TAA CAT TATA CC	50°C	257	[35]
SED; Staphylococcal enterotoxin D	CTA GTT TGG TAA TAT CTC CT	TAA TGC TAT ATC TTA TAG GG	50°C	317	[35]
SEE; Staphylococcal enterotoxin E	AGG TTT TTT CAC AGG TCA TCC	CTT TTT TTT CTT CGG TCA ATC	50°C	209	[35]
SEG; Staphylococcal enterotoxin G	AAG TAG ACA TTT TTG GCG TTC C	AGA ACC ATC AAA CTC GTA TAG C	55°C	287	[35]
SEH; Staphylococcal enterotoxin H	GTC TAT ATG GAG GTA CAA CAC T	GAC CTT TAC TTA TTT CGC TGT C	48.4°C	213	[35]
SEI; Staphylococcal enterotoxin I	GGT GAT ATT GGT GTA GGT AAC	ATC CAT ATT CTT TGC CTT TAC CAG	50°C	454	[35]
SEJ; Staphylococcal enterotoxin J	CAT CAG AAC TGT TGT TCC GCT AG	TGA ATT TTA CCA TCA AAG GTA C	50°C	142	[35]