Thrombin Maybe Plays an Important Role in MK Differentiation into Platelets

Figure 7

Particles released from Meg-01 cells after thrombin treatment are functional. Meg-01 cells were treated with thrombin (2 U/mL) or TPO (80 ng/mL) for 24 h; then the particles were concentrated by centrifugation. (a) Particles were incubated with AlamarBlue for 24 h at 37°C. The absorbance at 570 nm and 600 nm was measured. Each point represents mean ± SD of three replications. 570 (%) = [570 − (600 × 0)] × 100, where 0 = 570/600. (b) The expression of CD61 in platelet-like particles was assayed by flow cytometry (left curves: isotype controls; right curves: antibodies).

(a)

(b)