Bioguided Fraction and Isolation of the Antitumor Components from Phyllanthus niruri L.

Table 1

The IC₅₀ of Chang-liver and HCC cells treated with organic solvent-soluble fractions of P. niruri. Data represent Means ± SE from 3 independent experiments.

Components	IC50 (μg/mL)
	Chang-liver	SMMC7721	Bel7402
75% EtOH extract	>100	>100	71.86 ± 0.43
Petroleum ether fraction (Fr.1)	74.63 ± 7.68	68.49 ± 1.96	>100
CHCl3 fraction (Fr.2)	>100	84.42 ± 4.97	>100
EtOAc fraction (Fr.3)	69.03 ± 5.33	64.92 ± 6.04	58.16 ± 3.27
Fr.3.1	>100	40.72 ± 6.32	>100
Fr.3.2	83.68 ± 3.41	21.86 ± 0.60	27.80 ± 4.09
Fr.3.2.1	>100	59.63 ± 18.34	44.20 ± 13.55
Fr.3.2.2	>100	63.39 ± 5.59	67.27 ± 7.23
Compound 1	73.49 ± 19.18	35.89 ± 3.99	24.31 ± 0.66
Compound 2	93.35 ± 11.99	14.82 ± 1.00	15.52 ± 4.25
Fr.3.3	>100	30.28 ± 0.33	34.88 ± 2.00
Fr.3.4	>100	41.52 ± 0.46	57.41 ± 0.34
Fr.3.5	76.98 ± 6.32	50.73 ± 9.86	56.76 ± 8.70
Fr.3.6	70.70 ± 12.67	>100	>100
Fr.3.7	73.20 ± 6.51	>100	>100
n-BuOH fraction (Fr.4)	96.07 ± 10.94	68.55 ± 6.07	54.25 ± 3.73
Water fraction (Fr.5)	>100	>100	>100