Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

Figure 3

Sanger sequencing results (reverse complement) of PCR products including UGT1A128 A(TA)6/7TAA and UGT1A16 G>A. (a) Sequencing by the primer SP6. (b) Sequencing by the primer SP7. (c) and (d) were two haplotypes obtained from TA cloning and Sanger sequencing. The arrows indicated the loci tested.

(a) Homozygote A(TA)7TAA

(b) Heterozygote A(TA)6/7TAA

(c) H1:G-(TA)7

(d) H2:A-(TA)6