Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

<div>Pyrosequencing results of the primers were selectively blocked with ddTTP in exo<sup>−</sup> Klenow polymerase. (a) Sequencing by the primer SP1 and synthesized template T1. (b) Sequencing by the primer SP1 and synthesized template T2. (c) Sequencing by the primer SP1 and an equal mixture of templates T1 and T2. (d) Sequencing by the primer SP1 and synthesized template T1 without adding ddTTP as a nonblocking control.</div>

BioMed Research International

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Figure 5

Figure 5: Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension 