Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

Figure 6

Pyrosequencing and Sanger sequencing results of PCR products including K1637K A>G and S1647T T>A. The red arrows indicated a ddNTP-blocked site. The black arrows represented SNP loci. The PCR products sequenced by the primer SP2 with the blocking of (a) ddGTP and (b) ddATP, respectively. (c) Two haplotypes obtained from TA cloning and Sanger sequencing.

(a)

(b)

(c)