Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

Table 2

Synthesized sequences for verifying the feasibility of ddNTP blocking efficiency.

Name	Nucleotide sequence (5→3)
T1	Biotin-TAAAATACTGTGACATGTAGTTCTTTGGAAATTTCCTTTTTTTTGAAAGAAATTTTTCCACATCTCTACGCGAAA
T2	Biotin-TAAAATACTGTGTCATGTAGTTCTTTGGAAATTTCCTTTTCTTTGAAAGAAATTTTTCCACATCTCTACGCGAAA
SP1	AAAT TTCCAAAGAA CTACATG

T1 and T2 represented synthesized haplotypes related to K1637K and S1647T. The italic segments were the polymorphic sites, and the underlined segments were the regions that hybridized with the sequencing primer SP1.