Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

Table 3

Synthesized sequences used for genotyping and haplotyping.

Genotype	PCR primer sequences (5′3′)	Typing primer sequences (5′3′)	Typing primer applications
S1647T; (T>A)	TF: TGACAGTGAAAGTGGAAGGTTG	SP1: AAATTTCCAAAGAACTACATG	Genotyping
K1637K; (A>G)	TR: Biotin-CCTATTGGCAAAGCAATCTGGA	SP2: TGTGGAAAAATTTCTTTCAAA	Genotyping; haplotyping
		SP3: TGTGGAAAAATTTCTTTCAAAG	Haplotyping
UGT1A128; [A(TA)6TAA>A(TA)7TAA]	GF: Biotin-CCCTGCTACCTTTGTGGACT	SP4: GGTTCGCCCTCTCCTACTTA	Genotyping
UGT1A16; (G>A)	GR: CATTATGCCCGAGACTAACAAA	SP5: GTCTTCAAGGTGTAAAATGCTC	Genotyping; haplotyping
		SP6: GTCTTCAAGGTGTAAAATGCTCC	Haplotyping
		SP7: GTCTTCAAGGTGTAAAATGCTCT	Haplotyping

The SNP sites associated with typing primers were as follows. S1647T: SP1; K1637K: SP2 and SP3; UGT1A128: SP4; UGT1A16: SP5, SP6, and SP7. on ddNTP blocking. on allele-specific primer extension.