Expression Analysis of Hairpin RNA Carrying Sugarcane mosaic virus (SCMV) Derived Sequences and Transgenic Resistance Development in a Model Rice Plant

Figure 1

Schematic diagrams representing the chimeric CP:Hc-Pro hairpin RNA construct Ubi-hpCP:Hc-Pro (a) and the target reporter gene construct 35S-GUS:CP:Hc-Pro (b). 35S, Cauliflower mosaic virus 35S promoter; Ubi, maize ubiquitin promoter; HPT, hygromycin phosphate transferase gene; OCS Ter, Agrobacterium octopine synthase gene terminator sequence; Nos Ter, Agrobacterium nopaline synthase gene terminator; CAT INTRON, first intron of castor bean catalase-1 gene; CRE INTRON, the third intron of the Aegilops tauschii go35 NBS-LRR (go35) gene; SpeI, restriction site used for Southern blot analysis; LB, T-DNA left border; RB, right border.

(a)

(b)