Research Article
Expression Analysis of Hairpin RNA Carrying Sugarcane mosaic virus (SCMV) Derived Sequences and Transgenic Resistance Development in a Model Rice Plant
Figure 3
Southern blot analysis of T0 transgenic rice plants. DNA extracted from transgenic rice leaves was digested with SpeI restriction enzyme, separated in 0.8% agarose gel, transferred to HyBond-N+ Nylon membrane, and hybridized with radioactive labeled CP:Hc-Pro DNA probe. Numbers 1–5 indicate five Ubi-hpCP:Hc-Pro transgenic lines. VC, transgenic rice plants containing the empty vector control transgene. M, DNA marker (1 kb plus Gene Ruler). The arrow indicates the single transgene band in lines 2, 3, and 4.