Expression Analysis of Hairpin RNA Carrying Sugarcane mosaic virus (SCMV) Derived Sequences and Transgenic Resistance Development in a Model Rice Plant

Figure 4

T₀ transgenic rice plants express 21–24 nt siRNAs. (a) Northern blot hybridization for the detection of siRNA from transgenic rice lines. Twenty-five μg of total RNA extracted from leaves of transgenic plants was hybridized with antisense CP:Hc-Pro RNA probe (the upper panel). The U6 small nuclear RNA was hybridized as a loading control (the lower panel). Lane M represents the 21–24 nt radiolabeled RNA size marker, with a less-exposed picture given on the left to clearly identify the 21 and 24 nt bands. VC is transgenic rice plants containing the empty vector control transgene while, Lanes 1–5 are the same five Ubi-hpCP:Hc-Pro transgenic rice lines shown in Figure 3. (b) Histogram representing the relative intensity of the sRNA band on the Northern blot above.

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