Expression Analysis of Hairpin RNA Carrying Sugarcane mosaic virus (SCMV) Derived Sequences and Transgenic Resistance Development in a Model Rice Plant

Figure 5

Ubi-hpCP:Hc-Pro transgene expression and SCMV resistance analysis in T₁ generation. (a) Northern blot hybridization of T₁ progeny of four independent Ubi-hpCP:Hc-Pro lines (#2, #3, #4, and #5) and an empty vector control line (VC) using ³²P-labelled antisense CP:Hc-Pro RNA as probe, which shows Ubi-hpCP:Hc-Pro expression from #2, #3 (except for one plant), #4, and one of #5 plants, but not from the vector control and the other three #5 plants. Lower panel shows RNA loading control. Asterisks indicate plants analysed in (c). (b) Vector control and Ubi-hpCP:Hc-Pro transgene T₁ transgenic rice plants inoculated six weeks before with SCMV. (c) Quantitative real-time RT-PCR analysis of SCMV accumulation in infected VC and hpRNA transgenic T1 plants using CP (left) or Hc-Pro (right) primers.

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