Early Production of the Neutrophil-Derived Lipid Mediators LTB₄ and LXA₄ Is Modulated by Intracellular Infection with Leishmania major

Figure 1

Infection with L. major leads to enhanced production of by neutrophils. PMN were coincubated with L. major stationary phase promastigotes for 300 min at 37°C in humidified atmosphere containing 5% CO₂ in complete medium. The multiplicity of infection (MOI) for L. major was 5. (a) The micrograph shows Diff Quik-stained neutrophils with internalized L. major promastigotes (arrows). (b) L. major-infected and uninfected neutrophils were treated with ionomycin (0.2 μM, 10 min). LTB₄ content of the supernatants was measured by ELISA. (c) L. major-infected and uninfected neutrophils were exposed to LPS (1 μg/ml, 30 min) followed by fMLP (0.5 μM, 10 min). LTB₄ content of the supernatants was measured by ELISA. , n.d.: not detectable, and .

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