Postembedding Decalcification of Mineralized Tissue Sections Preserves the Integrity of Implanted Biomaterials and Minimizes Number of Experimental Animals

Figure 5

Histological stains of undecalcified tibia with intramedullary pin aligned in compliance with decalcified bone. The bone samples were embedded in PMMA T7200. Masson-Goldner staining of undecalcified bone shows the presence of soft tissue around the implant region and the magnified image shows the clear view of osteoid (a–c). The decalcified bone section shows the comparative view and presence of soft tissue around the implant region (d–f). The magnified image shows the view of osteocyte canaliculi. Images (a) and (d) were acquired by 5x magnification objective; then individual tiles were stitched together by Leica application suite (LASX) software. Images (b) and (e) were acquired by 40x magnification objective. Images (c) and (f) were acquired by 100x magnification objective.

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