Postembedding Decalcification of Mineralized Tissue Sections Preserves the Integrity of Implanted Biomaterials and Minimizes Number of Experimental Animals

Figure 7

Chemical investigations show preserved collagenous portion before and after decalcification. (a) Complete depletion of mineral constituents within tissue and material. (b) Minerals masked the signal of organic compounds before decalcification; however, after decalcification the surface analysis depicts demineralized areas as cartilaginous portions. The overlay shows that portions before decalcification were highly preserved.

(a)

(b)