Interleukin-17 Can Induce Osteoarthritis in Rabbit Knee Joints Similar to Hulth’s Method

Figure 3

(a) The safranin O staining of cartilage shows that the control group exhibited consistent matrix staining, an integrated surface, and normal cellularity at all 5 time points. Similar to the control group, the 4 experimental groups exhibited no sign of degradation at 72 h. At 1 week, mildly reduced safranin O staining of the surface layer of the articular cartilage was seen in all groups except the control group, but cartilage injury or cellularity anomaly was not observed. At 3 weeks, 3 groups (the Hulth group, the 10-ng group, and the 50-ng group) exhibited significantly reduced safranin O staining at the surface of the articular cartilage, while the 1-ng group displayed mildly reduced safranin O staining. At 6 weeks and 12 weeks, the 3 groups showed histological signs of severe osteoarthritis compared with the control group, while the 1-ng group displayed obviously reduced safranin O staining but only mild cartilage injury. Green arrows indicate decreased staining, and red arrows indicate cartilage defects. (b) Mankin’s score of cartilage. Compared to the control group, , . ^#Comparing the 3 IL-17 groups to the Hulth group, , .

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