JianPi JieDu Recipe Inhibits Epithelial-to-Mesenchymal Transition in Colorectal Cancer through TGF-β/Smad Mediated Snail/E-Cadherin Expression

Figure 1

Inhibitory effect of JPJD on the EMT of TGF-β-stimulated LoVo cells. (a) Inhibitory effect of JPJD on the proliferation of LoVo cells. LoVo cells were treated with JPJD of different concentrations including 12.5 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 300 μg/mL, and 400 μg/mL. After 24 h, 48 h, and 72 h, CCK-8 assay was performed to investigate the growth inhibitory effect of JPJD on LoVo cells. (b) Morphological observation of the TGF-β-stimulated or JPJD treated LoVo cells. The concentration of TGF-β used in the whole research was 10 ng/ml. The concentrations of JPJDs were calculated according to CCK-8 results, including 12.5 μg/mL, 25 μg/mL, and 50 μg/mL. The magnification of the microscopic pictures was ×200. (c) Impact of JPJD on the protein expression of Vimentin and E-cadherin in TGF-β-stimulated LoVo cells. The time of TGF-β-stimulation and JPJD treatment was 48 hours. , versus control LoVo cells, and and , versus only TGF-β-stimulated cells.

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