JianPi JieDu Recipe Inhibits Epithelial-to-Mesenchymal Transition in Colorectal Cancer through TGF-β/Smad Mediated Snail/E-Cadherin Expression

Figure 3

Impact of JPJD on the invasion and metastasis of TGF-β-stimulated LoVo cells. (a) Transwell experiment was performed to observe the impact of JPJD on the invasion and metastasis of TGF-β-stimulated LoVo cells. The magnification of the microscopic pictures was ×100. (b) Numbers of invasive and migrated cells shown as mean ± SD, . (c, d, e) Real-time PCR, western blot, and ELISA were, respectively, performed to test the effect of JPJD on the MMP-2 and MMP-9 expression of TGF-β-stimulated LoVo cells, as well as the secretory levels of MMP-2/MMP-9 in the culture medium of TGF-β-stimulated LoVo cells. Here, JPJD-L is 12.5 μg/mL, JPJD-M is 25 μg/mL, JPJD-H is 50 μg/mL, and the time of TGF-β-stimulation and JPJD treatment was 48 hours. , versus control LoVo cells, and and , versus only TGF-β-stimulated cells.

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