Effects of Poly(ADP-Ribose) Polymerase-1 Inhibition in a Neonatal Rodent Model of Hypoxic-Ischemic Injury

Figure 4

TUNEL staining revealed increased neurodegeneration after HI in the dentate gyrus (DG) and cortex (Cx) compared to sham controls receiving vehicle (a). Images of male animals receiving hypothermia (HT), TES448 only, or a combined therapy suggest a neuroprotective effect of PARP inhibition and cooling therapy after HI (a) although statistical significance could not be determined (b). Female rat pups demonstrated an increase of total TUNEL immunopositive cells after a combined treatment suggesting a gender-dependent effect of PARP inhibition and hypothermia ((a) and (c)). Values of total TUNEL-positive cells are presented as box plots including median values, the 25% and the 75% percentile, one-way ANOVA with Bonferroni’s multiple-comparison test, males = 5–11 per group, and females = 7–11 per group.

(a)

(b)

(c)