Curcuma longa Is Able to Induce Apoptotic Cell Death of Pterygium-Derived Human Keratinocytes

Figure 6

Immune fluorescence labelling of human keratinocytes derived from pterygium explants after treatment with BCA and Curcuma longa (CL) for 24 h. A significantly increased positivity for the terminal deoxynucleotidyl transferase- (TdT-) mediated nick end labelling (TUNEL) was found in samples treated with BCA (%). In Curcuma longa-treated samples a different pattern of apoptosis was revealed due to the presence of DNA also in the cytoplasm (%). Red arrow heads indicate some apoptotic cells. Nuclei were counterstained with 6-diamino-2-phenylindole (DAPI) (blue fluorescence). Green and blue fluorescence single emissions are overlapped in the merge panels. Original magnification: 40x. Images displayed are representative of 3 independent experiments.