Genetic Association Study of KCNQ5 Polymorphisms with High Myopia

Table 1

Information of primers and probes for unlabeled probe melting curve analysis.

SNP	Forward primers (5′3′)	Reverse primers (5′3′)	Unlabeled probes (5′3′)
rs9342979	GCCTGGTAGACAGACAAA	ACCTCTGGGCAAGTTAT	GCTCTCAAGATACAGTATATGTTC-Phos
rs9351953	GTAAGAGGGTCCCAAGTAAA	CAGTGAGATCCAAGGTTGAA	AAACTGCTTAAGGGCAATTGCTGC-Phos
rs3920868	GCCTTCTAGCTGGTAAGAG	TCTCTCCCTTTCTGAGGTG	GCTGCAAGAAAATAATAAAATTGGGTGA-Phos
rs7775087	TATTATAGTCATCTTAGGAGGT	CTTCCCATCGTTACCCAA	ACATGAAAAGCAAGAAGAAGAGCC-Phos
rs7744813	ACCTTGCTTCTAACCATATC	TTAGGTTTATGGAGTGGAAG	CAAGTAGAATGAATTACCTGTGACAGATGAC-Phos

Limiting primer; Phos, probes are phosphorylated at the 3′ end to prevent extension by DNA polymerase.