Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis

Figure 1

Expression and confirmation of F17A protein. (a) F17A protein was produced under the influence of IPTG in expression host E. coli BL21 (DE3). The expressed protein was Ni-NTA purified and run on gradient SDS-PAGE gel and immunoblotted against anti-His antibody. A dominant band of ~20 kDa of the expected size is indicated as recombinant F17A protein. (b) F17A protein run on SDS-PAGE and stained indicated by the expected size of ~20 kDa. Line M: protein marker; line 1: F17A protein.

(a)

(b)