Research Article

Autoantibodies against CYP-2C19: A Novel Serum Marker in Pediatric De Novo Autoimmune Hepatitis?

Table 1

Studies investigating LM and LKC autoantibodies with more than one immunological assay in patients affected by AIH of different etiologies. Studies are ordered by year of publication.

ReferenceYearDiagnosisAbAssay: IF on
liver/kidney
Assay other than IFTarget Ag (kD)Age of patients

Bourdi et al. [17]1990AIH-drug (dihydralazine induced)LMLiver [+]
Kidney [−]
IB, rat [−]
IB, human [+]
CYP-1A2
(~52 kD)
Adult

Sacher et al. [9]
Manns et al. [10]
1990AIH-APS-1LMLiver [+]
Kidney [−]
IB, human [+]CYP-1A2
(~52 kD)
Pediatric

Clemente et al. [11, 12]1997AIH-APS-1LMLiver [+]
Kidney [−]
IB, rat [−]
IB, human [+]
CYP-1A2
(~52 kD)
Pediatric

Gebre-Medhin et al. [13]1997AIH-APS-1LMLiver [+]
Kidney [−]
IB, human [+]CYP-1A2
(~52 kD)
Pediatric

Obermayer-Straub [14]2001AIH-APS-1LMLiver [+]
Kidney [−]
IB human CYPsCYP 1A2Adult and pediatric

Aguilera et al. [6]2001Post-OLT AIHLKCLiver [+]
kidney [+]
Human liver
cDNA expression library [+]
GSTT1
(~29 kD)
Adult

Salcedo et al. [18]2002Post-OLT AIHLKCLiver [+]
kidney [+]
ID, rat [+]
Human not tested
Cytosol Ag not studiedAdult

Huguet et al. [7]2007Post-OLT AIHLKC
Liver [+]
Kidney [+]
2D-IB
Rat [+]
Human not tested
Cytosol Ag = GSTT1
(~25 kD)
Adult

Meloni et al. [16]2012AIH-APS-1LMLiver [+]
Kidney [−]
IB human CYPsCYP1A2Pediatric

Clemente et al.Present studyPost-OLT AIHLMLiver [+]
Kidney [−]
IB, rat [−]
IB, human [+]
CYP-2C19
(55,9 kD)
Pediatric

Ag, antigen; AIH, autoimmune hepatitis; AIH-drug, drug-induced AIH; AIH-APS-1, Type 1 Polyglandular Syndrome associated AIH; CYP, cytochrome P450; GSTT1, glutathione-S-transferase T1; IB, immunoblotting; 2D-IB, two-dimensional immunoblotting; ID, immunodiffusion; IF, immunofluorescence; kD, kilodaltons of Ag molecular weight; LKC, liver kidney cytosol antibody; LM, liver microsomal antibody; LKM, liver and kidney microsomal antibody; post-OLT AIH, postorthotopic liver transplantation “de novo” AIH; Pts, patients.