BioMed Research International / 2017 / Article / Tab 1 / Research Article
Autoantibodies against CYP-2C19: A Novel Serum Marker in Pediatric De Novo Autoimmune Hepatitis? Table 1 Studies investigating LM and LKC autoantibodies with more than one immunological assay in patients affected by AIH of different etiologies. Studies are ordered by year of publication.
Reference Year Diagnosis Ab Assay: IF on liver/kidney Assay other than IF Target Ag (kD) Age of patients Bourdi et al. [17 ] 1990 AIH-drug (dihydralazine induced) LM Liver [+ ] Kidney [−] IB, rat [−] IB, human [+ ] CYP-1A2 (~52 kD) Adult Sacher et al. [9 ] Manns et al. [10 ] 1990 AIH-APS-1 LM Liver [+ ] Kidney [−] IB, human [+ ] CYP-1A2 (~52 kD) Pediatric Clemente et al. [11 , 12 ] 1997 AIH-APS-1 LM Liver [+ ] Kidney [−] IB, rat [−] IB, human [+ ] CYP-1A2 (~52 kD) Pediatric Gebre-Medhin et al. [13 ] 1997 AIH-APS-1 LM Liver [+ ] Kidney [−] IB, human [+ ] CYP-1A2 (~52 kD) Pediatric Obermayer-Straub [14 ] 2001 AIH-APS-1 LM Liver [+ ] Kidney [−] IB human CYPs CYP 1A2 Adult and pediatric Aguilera et al. [6 ] 2001 Post-OLT AIH LKC Liver [+ ] kidney [+ ] Human liver cDNA expression library [+ ] GSTT1 (~29 kD) Adult Salcedo et al. [18 ] 2002 Post-OLT AIH LKC Liver [+ ] kidney [+ ] ID, rat [+] Human not tested Cytosol Ag not studied Adult Huguet et al. [7 ] 2007 Post-OLT AIH LKC Liver [+ ] Kidney [+ ] 2D-IB Rat [+] Human not tested Cytosol Ag = GSTT1 (~25 kD) Adult Meloni et al. [16 ] 2012 AIH-APS-1 LM Liver [+] Kidney [−] IB human CYPs CYP1A2 Pediatric Clemente et al. Present study Post-OLT AIH LM Liver [+] Kidney [−] IB, rat [−] IB, human [+] CYP-2C19 (55,9 kD) Pediatric
Ag, antigen; AIH, autoimmune hepatitis; AIH-drug, drug-induced AIH; AIH-APS-1, Type 1 Polyglandular Syndrome associated AIH; CYP, cytochrome P450; GSTT1, glutathione-S-transferase T1; IB, immunoblotting; 2D-IB, two-dimensional immunoblotting; ID, immunodiffusion; IF, immunofluorescence; kD, kilodaltons of Ag molecular weight; LKC, liver kidney cytosol antibody; LM, liver microsomal antibody; LKM, liver and kidney microsomal antibody; post-OLT AIH, postorthotopic liver transplantation “de novo” AIH; Pts, patients.