PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells

Figure 2

UVB irradiation downregulated PTEN expression and induced DNA damage in RPE cells. (a) Cells were exposed to UVB radiation (60 mJ/cm²) and then incubated for 0–24 h. (b) Cells were exposed to 0–90 mJ/cm² UVB and then incubated for 24 h. mRNA expression level of PTEN was analyzed by Real-Time PCR. The relative expression data were presented after normalization to GAPDH. (c) Following UVB exposure (0, 60, 90 mJ/cm²) in RPE cells for 24 hours. Protein levels were detected by western blot. α-Tubulin expression was detected as the loading control. (d, e) Bar graphs represent the relative PTEN and p53 protein levels. The ratio of PTEN was quantized by using Image J (, t test, , bars represent SD).

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