PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells

Figure 5

The overexpression of PTEN attenuated cell apoptosis and DNA damage induced by UVB irradiation. RPE cells were stably transfected with overexpression plasmid. Then cells were treated with UVB (90 mJ/cm²) for 24 h. Levels of the mRNA (a) and protein (b) expression were detected by Real-Time PCR and western blot. And DNA damage related protein p-H2A was detected. (c) Bar graphs represent the relative PTEN protein levels normalized to that of α-Tubulin of different groups. (d) Cells were stained with Multicaspase/7-AAD for quantitative measurement using flow cytometry. (e) A representative apoptosis along with the statistical data was shown. These results are expressed as mean ± SD from three experiments (, versus control).

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