Andrographolide Promotes Neural Differentiation of Rat Adipose Tissue-Derived Stromal Cells through Wnt/β-Catenin Signaling Pathway

Figure 2

Morphological changes during neural induction. ((a), (b)) After 3 days of differentiation, there were no differences in the percentage of neuron-like cells (42.9% in the control group and 43.27% in the ANDRO group). After 6 days (3 days after treatment with ANDRO) and 10 days (1 week after treatment with ANDRO) of differentiation, significant differences between the groups were observed in terms of the percentage (3 days: 50.5% in the control group and 59.77% in the ANDRO group; 10 days: 58.57% in the control group and 73.4% in the ANDRO group; ). Zoomed images of the black square ((c) and (e)) show neuron-like cells (red arrowhead); zoomed images of the black square ((d) and (f)) show connections (green arrowhead), control group: (c) and (d) and ANDRO group: (e) and (f). Scale bar: 200 μm.