Uric Acid Induces Endothelial Dysfunction by Activating the HMGB1/RAGE Signaling Pathway

Figure 2

A high concentration of UA (20 mg/dL) upregulates the expression of RAGE and HMGB1 in HUVECs, accompanied by an increase in released HMGB1. (a) UA significantly upregulated the mRNA expression RAGE and HMGB1 in a time-dependent manner. (b) UA significantly upregulated the protein expression of RAGE in a time-dependent manner, while the protein expression of HMGB1 decreased. (c) UA significantly increased release of HMGB1 protein into the extracellular fluid. Data are expressed as means ± SD, , versus 0 h group.

(a)

(b)

(c)